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Performance Data of
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The
tables below demonstrate that Poly Net's Polymer
Pro matrix runs faster and equals the quality of Amersham's
matrix. Clipped readlength is total number of bases called with
98% accuracy. Unclipped readlength is the total number of bases
called during the run.
Voltage=6.2kv
Run Time 150 min
Analysis of Phred 20+ By Polymer |
|
|
Number |
Mean |
Std
Dev |
Std
Err Mean |
Lower
95% |
Upper 95% |
| LPA
lot # 12446 |
46 |
608.457 |
96.950 |
14.295 |
579.67 |
637.25 |
| Poly
Net's Polymer Pro |
16 |
636.500 |
115.882 |
28.971 |
574.75 |
698.25 |
| Poly
Net's Polymer Pro |
16 |
616.813 |
83.373 |
20.843 |
572.39 |
661.24 |
| Poly
Net's Polymer Pro |
16 |
702.813 |
48.858 |
12.214 |
676.78 |
728.85 |
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Analysis
of CLIP readlength [98%] By Polymer
|
|
|
Number |
Mean |
Std
Dev |
Std
Err Mean |
Lower
95% |
Upper
95% |
| LPA
lot # 12446 |
46 |
660.174 |
118.785 |
17.514 |
624.90 |
695.45 |
| Poly
Net MHS |
16 |
722.500 |
68.792 |
17.198 |
685.84 |
759.16 |
| Poly
Net MHS |
16 |
708.750 |
63.877 |
15.969 |
674.71 |
742.79 |
| Poly
Net MHS |
16 |
796.438 |
52.989 |
13.247 |
768.20 |
824.67 |
pGTC plate
(BD ver 1.0) was run on MegaBACE: Conditions: matrix fill time
= 180 sec, sample injection time = 20 sec, run voltage = 6.2kv,
run time = 150 min
|
|
Analysis of UNCLIP Readlength
|
| |
Number |
Mean |
Std Dev |
Std Err Mean |
Lower 95% |
Upper 95% |
| MHS Poly Net |
48 |
761.625 |
193.801 |
27.973 |
705.35 |
817.90 |
| LPA lot # 13892 |
48 |
644.938 |
127.381 |
18.386 |
607.95 |
681.93 |
|
&npsp;
|
|
Analysis of Phred 20+ By Polymer
|
| |
Number |
Mean |
Std Dev |
Std Err Mean |
Lower 95% |
Upper 95% |
| MHS Poly Net |
48 |
548.438 |
158.647 |
22.899 |
502.37 |
594.50 |
| LPA lot # 13892 |
48 |
545.250 |
131.620 |
18.998 |
507.03 |
583.47 |
In the graph below Poly Net is red and Amersham is blue. Impure is a measure of peak shape [Gaussian] and the existence of Phantom peaks. You will notice that Poly Net has virtually none. The data represents about 900 capillaries runs. Good Bases are Phred scored at 20 and above.
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